Diagnostic Hybrids

Sensitive and Enterovirus Specific Identification Stain

• Superior Staining with newly developed monoclonal antibodies
• A blend of Monoclonal Antibodies to assure broad Enterovirus coverage
• Antibodies targeting the VP1 Protein allow for Enterovirus-specific identification
• Clear staining results without a blocking reagent

Simplifies Workflow

• No additional steps required to report results after simple IFA staining procedure is completed
• Super E-Mix† combines BGMK-hDAF* and A549 cells to allow isolation of enterovirus in a single culture system
• Complete Kit Format reduces QC Time
• Now available in a frozen ReadyCell format - ready for use in 4 minutes

Rapid Final Results For All Patients

• Results in as little as 16 hours
• Totally Tubeless cell format simplifies cell management
• No “Presumptive” positive classification for rapid final results without additional follow up

Product Numbers

01-050000     D³ IFA Enterovirus ID Kit
Includes: Enterovirus Mab Reagent (5-mL), Anti-Mouse FITC Conjugate (5-mL), 40x PBS (25-mL), Mounting Fluid (7-mL), Five Control Slides (negative, Entero 70, Coxsackie A9, Coxsackie B2, Echo 5)

Individual Components

01-050001     D³ IFA Enterovirus Reagent DuoPack (5-mL Enterovirus MAb reagent, 5-mL Anti-Mouse FITC Conjugate)
01-00080       Enterovirus Control Slides (10)

Educational Resources

• A Multiyear History of Cell Culture During Conversion to Totally Tubeless Virology and Effect on Isolation Rates of Viruses
  

  Phyllis McCurdy and James W. Snyder. University of Louisville School of Medicine and Hospital, Louisville, KY. Download File

• Comparison of Culture for Cytopathic Effect to Shell Vial and Fluorescent Antibody Stain for Viral Diagnosis
  

  K. Wilkins, H. McIntire, and J. Coleman. Department of Pathology, University of Mississippi Medical Center, Jackson, MS. Download File

• Comparison of Diagnostic HYBRIDS D³ IFA Enterovirus Identification Kit to Millipore Light Diagnostics Pan-Enterovirus Reagent
  

  J. Langer¹, C.A. Petti^1,2. ¹ARUP Laboratories, Inc, Salt Lake City, UT, and ²Dept. of Pathology, University of Utah School of Medicine, Salt Lake City, UT. Download File

• Comparison of Sensitivity and Specificity of a New Enterovirus Group Reactive Monoclonal Antibody Reagent from Diagnostic HYBRIDS to Reagents from Chemicon and Dako
  

  Maria Vu, Elaine Yeh, David Schnurr. Viral and Rickettsial Disease Laboratory, California Department of Health Services, Richmond CA. Download File

• Development of Diagnostic Pan-Enterovirus Monoclonal Antibodies Using VP1 Recombinant Proteins
  

  Lynn Yihong Miao, Christina Pierce, Sarah Peaslee, Carl Shaw, April Brandon, Nate Chapman, Jill DeLotell, Jimmy Page, and David Scholl, Ph.D. Diagnostic HYBRIDS, Athens, OH. Download File

• Evaluation of the Diagnostic HYBRIDS D³ IFA Enterovirus Identification Kit for Culture Confirmation of Enteroviruses
  

  Joseph Jollick, Jeff Houtz, and Steve Ewers. Diagnostic HYBRIDS, Athens, Ohio. Download File

• Impact of Converting from Conventional Tube Cultures to Shell Vials: A Community Hospital Laboratory's Experience
  

  D. Stevenson, J.Hoffman, K.W. Beckman, and E. Matthews. Borgess Medical Center, Kalamazoo, MI. Download File

• Integration of Rapid Cell Culture and Molecular Analysis in a Clinical Virology Laboratory
  

  R. Wheeler, D. Hathaway, T. Hayes, D. Jaskot, J. Krahn, A. Tirolese, R. Lannigan. Virology Department, London Laboratory Services Group, London, Ontario. Download File